In this article by Patterson et al [Patterson BK, Francisco EB, Yogendra R, et al. Persistence of SARS CoV-2 S1 protein in CD16+ monocytes in post-acute sequelae of COVID-19 (PASC) up to 15 months post-infection. Front Immunol. 2022 Jan 10;12:746021. doi: 10.3389/fimmu.2021.746021.], it might be possible that integration occurred in this individual. Whether it was due to shedding or not remains to be determined.
For patients with IgG spike antibody titres at or above a dilution of 1/25,000 (BTW is this IgG1/3 or IgG2/4? and does it matter?), this might be an indication of integration of the vaccine derived nucleotide sequences into genomic DNA (i.e., the dreaded genetic modification of humans). Instead of trying to biopsy each organ in the body, we could purify genomic DNA from plasma. The contents of recently lysed cells (say the ones expressing the vaccine sequence for spike which are killed by the immune system in the presence of ivermectin and zinc) could be examined for gene copy number for the spike gene and for HERV-K102 [see my real time PCR method used in Laderoute MP et al, Open AIDS Journal, 2015 on how to run the tests for genomic DNA and exclude cDNA of the HERV-K102 particles. If HERV-K102 copy number is elevated (for example in people with high levels of spike antibody and not in those with little or no antibody and there is no active SARS-CoV-2 infection, this might imply that the exosomes associated with shedding might be the 100 nm HERV-K102 particles. In this case, one would like to also examine genomic DNA in macrophages/monocytes and in the bone marrow progenitors of the monocytes/macrophages. It is possible that integration might more commonly occur as a direct consequence of shedding (from HERV-K102 particles produced in sebocytes found in sebaceous glands in the mucosa carrying the RNA or DNA sequence of spike).
Of course shedding to the ever vaccinated from the ever vaccinated also occurred. The problem here is how to determine what pathology related to the direct inoculation of the Pfizer mRNA vaccine versus shedding and of course that due to breakthrough infections.
Dr Pierre Kory uses the levels of spike antibody as a surrogate marker for shedding, such as in discordant couples (one recently vaccinated, the other not, but the latter showing some symptoms). Some of these antibody levels reach extreme levels, making one ponder if there was an integration event in the new host but not in the original shedder ? I hate to say it but, if the vaccine LNPs get targeted to sebocytes in sebaceous glands in the upper respiratory tract (URT) in the vaccinated, the resulting HERV-K102 particles (around 100 nms which is mid-size for exosomes) that undergo reverse transcription and which carry integrase and become integrated, would be released in saliva and in aerosols. Upon absorption in the URT, lungs, skin or other types of mucosa of the new host, this could set up spike continuous manufacturing related to integration. If the cell type is a macrophage/sebocyte, it likely would not express spike (or HERV-K102 envelope) at its cell surface so it would be refractory to elimination. Spike protein then causes clotting/low zeta potential in the circulation (sudden death) and/or induces immunosenescence which favors cardiovascular disease and cancers. So in this hypothetical scenario, integration is more likely upon shedding to new host which fits with the very high levels of spike antibody as sometimes observed by Dr. Kory. To fix the problem, probably need to use a cocktail of alpha-fetoprotein antagonists: IVERMECTIN (or possibly Artemisinin/Artesunate), zinc, isoflavonoids (genistein), ensure vitamin D levels are well over 50 ng/ml, and in the USA could also include 7 keto-DHEA. Raises the issue that integration of spike sequences into genomic DNA may be more common relating to shedding, so the recently vaccinated may not be the population that would be most informative for possible integration. Thoughts?
Evidence that spike antibody isotypes seem to have enhanced the risk of death in the unvaccinated implies a mechanism of death pertained at least to the targeting of foamy macrophages and induction of immunosenescence in the old and new host. It is noteworthy that evidence of transfer of spike specific antibodies via shedding has been shown [ Kedl RM et al, 2023, Immunohorizons].
I wonder about morbidity and mortality spiking among the UK "unvaccinated" at the same time as amongst the "vaccinated".
We do know that in the US a whole lot of "vaccinated" people, admitted to hospitals with COVID, were entered as "unvaccinated", unless they had received a second dose more than 2 weeks prior AND that information was Already entered in the medical records at That Hospital.
I don't know exactly the methodology used to classify "vaccinated" and "unvaccinated" in the UK, but some of that same kind of misclassification bleed-over may have been at play.
In this article by Patterson et al [Patterson BK, Francisco EB, Yogendra R, et al. Persistence of SARS CoV-2 S1 protein in CD16+ monocytes in post-acute sequelae of COVID-19 (PASC) up to 15 months post-infection. Front Immunol. 2022 Jan 10;12:746021. doi: 10.3389/fimmu.2021.746021.], it might be possible that integration occurred in this individual. Whether it was due to shedding or not remains to be determined.
For patients with IgG spike antibody titres at or above a dilution of 1/25,000 (BTW is this IgG1/3 or IgG2/4? and does it matter?), this might be an indication of integration of the vaccine derived nucleotide sequences into genomic DNA (i.e., the dreaded genetic modification of humans). Instead of trying to biopsy each organ in the body, we could purify genomic DNA from plasma. The contents of recently lysed cells (say the ones expressing the vaccine sequence for spike which are killed by the immune system in the presence of ivermectin and zinc) could be examined for gene copy number for the spike gene and for HERV-K102 [see my real time PCR method used in Laderoute MP et al, Open AIDS Journal, 2015 on how to run the tests for genomic DNA and exclude cDNA of the HERV-K102 particles. If HERV-K102 copy number is elevated (for example in people with high levels of spike antibody and not in those with little or no antibody and there is no active SARS-CoV-2 infection, this might imply that the exosomes associated with shedding might be the 100 nm HERV-K102 particles. In this case, one would like to also examine genomic DNA in macrophages/monocytes and in the bone marrow progenitors of the monocytes/macrophages. It is possible that integration might more commonly occur as a direct consequence of shedding (from HERV-K102 particles produced in sebocytes found in sebaceous glands in the mucosa carrying the RNA or DNA sequence of spike).
Of course shedding to the ever vaccinated from the ever vaccinated also occurred. The problem here is how to determine what pathology related to the direct inoculation of the Pfizer mRNA vaccine versus shedding and of course that due to breakthrough infections.
Dr Pierre Kory uses the levels of spike antibody as a surrogate marker for shedding, such as in discordant couples (one recently vaccinated, the other not, but the latter showing some symptoms). Some of these antibody levels reach extreme levels, making one ponder if there was an integration event in the new host but not in the original shedder ? I hate to say it but, if the vaccine LNPs get targeted to sebocytes in sebaceous glands in the upper respiratory tract (URT) in the vaccinated, the resulting HERV-K102 particles (around 100 nms which is mid-size for exosomes) that undergo reverse transcription and which carry integrase and become integrated, would be released in saliva and in aerosols. Upon absorption in the URT, lungs, skin or other types of mucosa of the new host, this could set up spike continuous manufacturing related to integration. If the cell type is a macrophage/sebocyte, it likely would not express spike (or HERV-K102 envelope) at its cell surface so it would be refractory to elimination. Spike protein then causes clotting/low zeta potential in the circulation (sudden death) and/or induces immunosenescence which favors cardiovascular disease and cancers. So in this hypothetical scenario, integration is more likely upon shedding to new host which fits with the very high levels of spike antibody as sometimes observed by Dr. Kory. To fix the problem, probably need to use a cocktail of alpha-fetoprotein antagonists: IVERMECTIN (or possibly Artemisinin/Artesunate), zinc, isoflavonoids (genistein), ensure vitamin D levels are well over 50 ng/ml, and in the USA could also include 7 keto-DHEA. Raises the issue that integration of spike sequences into genomic DNA may be more common relating to shedding, so the recently vaccinated may not be the population that would be most informative for possible integration. Thoughts?
Evidence that spike antibody isotypes seem to have enhanced the risk of death in the unvaccinated implies a mechanism of death pertained at least to the targeting of foamy macrophages and induction of immunosenescence in the old and new host. It is noteworthy that evidence of transfer of spike specific antibodies via shedding has been shown [ Kedl RM et al, 2023, Immunohorizons].
I wonder about morbidity and mortality spiking among the UK "unvaccinated" at the same time as amongst the "vaccinated".
We do know that in the US a whole lot of "vaccinated" people, admitted to hospitals with COVID, were entered as "unvaccinated", unless they had received a second dose more than 2 weeks prior AND that information was Already entered in the medical records at That Hospital.
I don't know exactly the methodology used to classify "vaccinated" and "unvaccinated" in the UK, but some of that same kind of misclassification bleed-over may have been at play.